Reagents

Our laboratory has developed a number of transgenic lines that are available to the scientific community either through Jackson's labs (VE-Cadherin Cre-recombinase line) or via a standard UCLA MTA. To request the MTA please e-mail lmzhao@ucla.edu.

Transgenic lines:

Constitutive VE-Cadherin Cre-Recombinase

• Publication characterizing the model:
  
  Alva, J.A., Zovein, A.C., Monvoisin, A., Murphy, T., Salazar, A., Harvey, N.L., Carmeliet, P., Iruela-Arispe M.L. 2006. VE-Cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells. Dev Dyn. 235(3):759-767.
  
  
• Protocol for genotyping
  
• Resulting publications using this line
  
  Lee, S., Chen, T.T., Barber, C., Jordan, M. C., Murdock, J.; Desai, S., Ferrara, N., Nagy, A., and Iruela-Arispe, M.L. 2007. Aurocrine VEGF signaling is required for vascular homeostasis . Cell, 130:690-703
  
  

Inducible VE-Cadherin Cre-Recombinase

• Publication characterizing the model:
  
  Monvoisin, A., Alva, J.A., Hofmann, J.J., Zovein, A.C., Lane, T.F., and Iruela-Arispe, M.L. 2006. VE-cadherin-CreER(T2) transgenic mice: A model for inducible recombination in the endothelium. Dev. Dyn. 235:3413-3422.
  
  
• Protocol for genotyping
  
• Protocols for tamoxifen-induction
  
• Resulting publications using this line
  
  Hellstrom, M., Phng, L.K., Hofmann, J.J., Wallgard, El., Coultas, L., Limdblom, P., Alva, J., Nilsson, L., Gaiano, N., Yoon, K., Rossant, J., Iruela-Arispe, M.L., Kalen, M., Gerhardt, H., and Betsholtz, C. 2007. Dll4/Notch1 signaling is required for selection of single endothelial tip cells. Nature, 445:776-780.
  
  

Floxed progesterone receptor

• Publication characterizing the model:
  
  Hashimoto-Partyka, M., Lydon, J., and Iruela-Arispe, M.L. 2006. Generation of a mouse for conditional excision of progesterone receptor (PRCE). Genesis, 44:391-395.
  
  
• Protocol for genotyping
  
• Resulting publications using this line
  
   
  
  

ADAMTS1 knock-out

• Publication characterizing the model:
  
  Lee, N.V., Sato, M., Annis, D.S., Loo, J.A., Wu, L., Mosher, D.F. and Iruela-Arispe, M.L. 2006. ADAMTS1 mediates the release of anti-angiogenic polypeptides from TSP1 and TSP2. The EMBO J. 25:5270-5283.
  
  
• Protocol for genotyping
  
• Resulting publications using this line
  
  Krampert, M., Kuenzle, S., Thai, S.N., Lee, N., Iruela-Arispe, M.L., Werner, S. 2005. ADAMTS1 proteinase is upregulated in wounded skin and regulates migration of fibroblasts and endothelial cells. J Biol Chem. 280(25):23844-23852