Using 454 Sequencing to Survey The Transcriptome of Soybean Seeds
Containing Globular-Stage Embryos

We carried out a pilot study using the high-throughput 454 sequencing (454 Life Sciences) to survey the transcriptome of a globular-stage soybean seed. ~400,000 reads were generated with the average length of 200 bases in one run representing a deep sampling of the globular-stage seed transcriptome. Raw unprocessed EST sequences generated from this study have been submitted to Genbank Short Read Archive (SRA) division under accession number SRA001022. The processed EST sequences also have been submitted to Genbank dbEST division under accession numbers FK265369 to FK668879.

Click here to learn more about this study and download 454 EST sequences

Updated Soybean GeneChip Array Annotation

We annotated the Affymetrix Soybean Genome GeneChip Array using information from TAIR, TIGR, and Peking Transcription Factor databases. The current version of annotation was updated as of October 2007. Please check the link below to access the annotation and detailed description.

Click here to download our new annotation file and detailed description.

NSF Single Cell Genome Profiling Project in Soybean

We used laser capture microdissection to isolate specific compartments, tissues, and cell types from soybean seeds at different stages of development Ð including the seed coat, endosperm, and embryo regions and organ systems. Microarray, EST sequencing, and qRT-PCR experiments are carried out in order to determine (1) the spectrum of genes and processes that are active in different parts of the seed and (2) how transcription factors are partitioned within a seed. Functional studies are also carried out in order to determine what role compartment and tissue-specific transcription factors play in soybean seed development. Data generated from this NSF-sponsored project are continuously updated in our interactive relational seed database -- Gene Networks in Seed Development. Please check out this web site for up-to-date information about this project.

Click here to browse our database - Gene Networks in Seed Development.

Genomic Profiling of Arabidopsis Plant Life Cycle Using Affymetrix GeneChips

Utilizing a genomics approach, we profiled mRNAs active during Arabidopsis seed development using whole-genome Affymetrix GeneChips ATH1-121501. Specifically, we profiled mRNAs active during seed development at 24 hour post-fertilization, 7-8 days after pollination (DAP), 13-14 DAP, and 18-19 DAP. These gene sets were compared with those active in pre-fertilization ovules, seedlings 3 days after imbibition, leaves, roots, stems, and floral buds of the mature plant. Collectively, we have carried out whole-genome analysis of genes active during the entire Arabidopsis life cycle.

Click here to browse our Arabidopsis GeneChip Database

Posted by Brandon

8-23-05

Scarlet Runner Bean Embryo EST Project

We have identified ~20,000 ESTs representing messages active 6-7 days after fertilization in different regions of early embryos. See a catalog of embryo-proper and suspensor ESTs found to date.

Click Here to See a Functional Category Summary Table of the ESTs Identified

We've also created a database where you can query your sequences against our Scarlet Runner Bean ESTs using local BLAST tools.

Click Here to BLAST Your Sequences Against our Scarlet Runner Bean EST Sequences.

All of our EST sequences have been submitted to GenBank. Their accession numbers are: CA896559 to CA916678. You can access all of these sequences at NCBI.

Click Here for Access to Scarlet Runner Bean ESTs in the GenBank.

Posted by Brandon

8-23-05

BOB GOLDBERG'S NOVEL UNDERGRADUATE SCIENCE EDUCATION PROGRAM
Funded by National Science Foundation

This program contains two courses: HC70A and HC70AL, which specifically targets non-science majors and teaches them about science.

Planting the Seeds of Discovery

As an HHMI Professor, he plans to design and teach a course that will show students who are not science majors the numerous ways science has an impact on society, for example, the social, legal and ethical issues that arise from emerging new genetic technologies. Read more

Howard Hughes Medical Institute's Million-Dollar Professors

What would you do with a million dollars? Twenty scientists at research universities across the nation have 20 different answers. They all share a goal, however: to make science more engaging for undergraduates. Read more

HHMI Program

Click here to enter and explore Bob Goldberg's undergraduate education program sponsored by HHMI Million-Dollar Professors program.

Los Angeles Times Daily News

December 5, 2004 -- During the next 50 years, we will need to produce more food than in the entire history of mankind. And we will need to do this on a rapidly shrinking amount of land that is suitable for agriculture...Read more

Bob Goldberg Elected into the National Academy of Science

UCLA plant biologist Robert B. Goldberg was elected today to the prestigious National Academy of Sciences "in recognition of his distinguished and continuing achievements in original research," the academy announced Tuesday...Read more

Los Angeles Times Interview by Greg Goldin
Robert Goldberg: Seeds of Contention

Sunday, September 2, 2001. UCLA molecular biologist Robert B. Goldberg wants to understand in plants what stem-cell researchers want to understand in humans: How the undifferentiated molecules of a seed develop from nearly identical dabs into highly specialized plant parts...Read more

History's Harvest: Where Food Came From

Keck Genomics Symposium

Foods for the Future: Genomics of Bioengineered Foods

Sponsored by the W. M. Keck Foundation. Offered in cooperation with the Office of Continuing Medical Education David Geffen School of Medicine at UCLA and UCLA Extension.

87th Faculty Research Lecture

The annual Faculty Research Lectures of the Univeristy of California, Los Angeles present to the public our most distinguished scholars. It is our purpose to accord them the high recognition that is their due, and further to give our faculty, students and the citizens of this community an opportunity to understand the scholarly achievements and points of view of those whom we honor.

Genetic Engineering in Agriculture: Super Plants for the 21st Century

Robert B. Goldberg
Professor of Molecular, Cell and Developmental Biology
Thursday, December 2, 1999
3:00 - 4:30 PM PST
(Live Webcast)