Research
We will use laser capture microdissection to isolate specific compartments, tissues, and cell types from soybean seeds at early stages of development – including the seed coat, endosperm, and embryo regions and organ systems. Microarray, EST sequencing, and qRT-PCR experiments will be carried out in order to determine (1) the spectrum of genes and processes that are active in different parts of seed and (2) how transcription factors are partitioned within a seed. Functional studies will also be carried out in order to determine what role compartment and tissue-specific transcription factors play in soybean seed development. Data section coming soon!! Check here for updates. |
Utilizing a genomics approach, we profiled mRNAs active during Arabidopsis seed development using whole-genome Affymetrix GeneChips ATH1-121501. Specifically, we profiled mRNAs active during seed development at 24 hour post-fertilization, 7-8 days after pollination (DAP), 13-14 DAP, and 18-19 DAP. These gene sets were compared with those active in pre-fertilization ovules, seedlings 3 days after imbibition, leaves, roots, stems, and floral buds of the mature plant. Collectively, we have carried out whole-genome analysis of genes active during the entire Arabidopsis life cycle. |
We have identified ~20,000 ESTs representing messages active 6-7 days after fertilization in different regions of early embryos. See a catalog of embryo-proper and suspensor ESTs found to date. Click Here to See a Functional Category Summary Table of the ESTs Identified We've also created a database where you can query your sequences against our Scarlet Runner Bean ESTs using local BLAST tools. Click Here to BLAST Your Sequences Against our Scarlet Runner Bean EST Sequences. All of our EST sequences have been submitted to GenBank. Their accession numbers are: CA896559 to CA916678. You can access all of these sequences at NCBI. Click Here for Access to Scarlet Runner Bean ESTs in the GenBank. |
NSF Single Cell Genome Profiling Project in Soybean
Genomic Profiling of Arabidopsis Plant Life Cycle Using Affymetrix GeneChips
Scarlet Runner Bean Embryo EST Project|
Overall Research Interests: Molecular
and Genetic Dissection of Plant Development
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My laboratory
has been investigating the molecular processes controlling the
development of specialized cells in higher plants. The
major questions that I have addressed in my research are ....
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The Seed Institute was established in order to carry out basic research in seed biology with the objective of uncovering the genes and processes required to "make a seed." The long-range goal of the Seed Institute, in partnership with Ceres, is to use new insights obtained on the mechanisms that control seed formation to improve seeds in major crop plants .... Read more |
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Recent papers on embryo and anther development. |
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The annual Faculty Research Lectures of the Univeristy of California,
Los Angeles present to the public our most distinguished scholars.
It is our purpose to accord them the high recognition that is
their due, and further to give our faculty, students and the
citizens of this community an opportunity to understand the scholarly
achievements and points of view of those whom we honor.
UCLA plant biologist Robert B. Goldberg was elected today to the prestigious National Academy of Sciences "in recognition of his distinguished and continuing achievements in original research," the academy announced Tuesday. To view other awards, Click here . |
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UCLA Challenge Magazine article by Ben Carey in 1998 describes the research and vision of Bob Goldberg. Robert Goldberg dreams of a postmillennial farming—with superraces of corn, wheat and rice, plants producing industrial oils in their leaves, crops that clone themselves. Yields enough to feed the hungriest continents. Can four-pound tomatoes and bell peppers the size of basketballs be far behind? Read more
To grant or not. Plant Cell 8, 346-347 (1996)
1996 Bob Goldberg's editorial on the difficulty of obtaining fund to maintain a productive research group. Many scientists worry about whether they will be able to continue their research efforts, and scientific carrers, in the face of uncertain funding and relatively low funding levels. The pressure on academics of all ranks is enormous. It is no longer "publish or perish." It is now "find grant money or perish even if you do publish.".... Read more Bob Goldberg's editorial looking at the establishment of gene cloning and the evolution of biology in: |
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"History's Harvest: Where Food Came From" |
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Explore
the history of agriculture and the evolution of the foods we
eat today. Go back to ancient Mexico and examine the predessor
of present day corn, the teosinte. Travel to Europe during the
time of the Green Revolution. Travel to modern-day India where
farming is an occupation not a lifestyle. Explore the concerns
regarding GMOs. |
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Invited lectures and seminars given in the past few years. |
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Journal Covers of Published Papers Click here to look at the gallery of journal cover images generated from this lab. |
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