Amander Clark
Phone: 310-794-4201
Office: 457 BSRB

Research Interests

Our overall goal is to understand the cell and molecular basis of germline cell differentiation and epigenetic reprogramming. My laboratory uses CRISPR/Cas9 gene editing technologies, next generation sequencing, pluripotent stem cells and mouse modeling to achieve this goal. Results from our work will provide a biological understanding of the cell and molecular basis of human life and child health, and potentially the foundation for a cell based therapy to overcome human infertility.

Selected Publications

Clark, AT*., Gkountela, G., Chen, D., Liu, W., Sosa, E., Sukhwani, M., Hennebold, JD, Oriwg, KE, "Primordial germ cells acquire transplantation potential by Carnegie Stage 23", Stem Cell Reports (2017).

Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT, "An integration-free visus-free rhesus macaque induced pluripotent stem cell line (iPSC90) from embryonic fibroblasts", Stem Cell Research 21: 5-8 (2017).

Nagaraj R, Sharpley MS, Chi F, Braas D, Zhou Y, Kim R, Clark AT, Banerjee U., "Nuclear localization of mitochondrial TCA cycle enzymes as a critical step in mammalian zygotic genome activation", Cell 168: 210-223 (2017).

O'Brien CM, Chy HS, Zhou Q, Blumenfeld S, Lambshead JW, Liu X, Kie J, Capaldo BD, Chung TL, Adams TE, Phan T, Bentley JD, Mckinstry WJ, Oliva K, Mcmurrick PJ, Wang YC, Rossello FJ, Lindeman GJ, Chen D, Jarde T, Clark AT, Abud HE, Visvader JE, Nefzger CM, Polo JM, Loring JF, Laslett AL., "New monoclonal antibodies to defined cell surface proteins on human pluripotent stem cells", Stem Cells 35: 626-640 (2017).

Patel S, Bonora G, Sahakyan A, Kim R, Chronis C, Langerman J, Fitz-Gibbon S, Rubbi L Skelton RJP, Ardehali R, Pellegrini M, Lowry WE, Clark AT, Plath K, "Human embryonic stem cells do not change their X inactivation status during differentiation", Cell Reports 18: 54-67 (2017).

Sahakyan A, Kim R, Chronis C, Sabri S, Bonora G, Theunissen TW, Kuoy E, Langerman J, Clark AT, Jaenisch R, Plath K, "Naive Human pluripotent stem cells model X chromosome Dampening and X inactivation", Cell Stem Cell 20: 87-101 (2017).

Masaeli M, Gupta D, O'Byrne S, Tse HT, Gossett DR, Tseng P, Utada AS, Jung HJ, Young S, Clark AT, Di Carlo D., "Multiparameter mechanical and morphometric screening of cells", Scientific Reports 6: (2016).

Hargan-Calvopina J, Taylor S, Cook H, Hu Z, Lee SA, Yen M-R, Chiang, Y-S, Chen P-Y and Clark AT, "Stage-specific demethylation in primordial germ cells safeguards against precocious differentiation", Developmental Cell 39: 75-86 (2016).

Li S, Yen L, Pastor W, Johnston JB, Du J, Shew CJ, Liu W, Ho J, Stender B, Clark AT, Burlingame AL, Daxinger L, Patel DJ, Jacobsen SE, "Mouse MORC3 is a GHKL ATPase that localizes to H3K4me3 marked chromatin", PNAS 113: 108-116 (2016).

Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT, "An integration-free visus-free rhesus macaque induced pluripotent stem cell line (iPSC89) from embryonic fibroblasts Stem Cell Research", Stem Cell Research 17: 444-447 (2016).

Pastor WA, Chen D, Liu W, Kim R, Sahakyan A, Lukianchikov A, Plath K, Jacobsen SE, Clark AT, "Naive Human Pluripotent Cells Feature a Methylation Landscape Devoid of Blastocyst or Germline Memory", Cell Stem Cell 18: 323-329 (2016).

Clark, AT and Zwaka TP, "Editorial overview: Cell reprogramming, regeneration and repair: Reprogramming: the eternal circle", Current Opinion in Genetics and Development 34: v-vi (2015).

Clark, AT, "DNA methylation remodeling in vitro and in vivo", Current Opinion in Genetics and Development 34: 82-87 (2015).

Chen, D., Clark, AT, "Human germline differentiation charts a new course", EMBO J 34: 975-977 (2015).

Oliveros-Etter M, Li Z, Nee K, Hosohama L, Hargan-Calvopina J, Lee, SA, Joti P, Yu J Clark AT, "PGC Reversion to Pluripotency Involves Erasure of DNA Methylation from Imprinting Control Centers followed by Locus-Specific Re-methylation", Stem Cell Reports 5: 337-349 (2015).