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Amander Clark
Phone: 310-794-4201
Office: 457 BSRB

Research Interests

Our overall goal is to understand the cell and molecular basis of germline cell differentiation and epigenetic reprogramming. My laboratory uses CRISPR/Cas9 gene editing technologies, next generation sequencing, pluripotent stem cells and mouse modeling to achieve this goal. Results from our work will provide a biological understanding of the cell and molecular basis of human life and child health, and potentially the foundation for a cell based therapy to overcome human infertility.

Selected Publications

1. Pastor, WA., Liu, W., Chen, D., Ho, J., Kim, R., Hunt, T., Lukianchikov, A., Liu, X., Polo, JM., Jacobsen, SE., Clark, AT, "TFAP2C regulates transcription in human naive pluripotency by opening enhancers", Nature Cell Biology 20: 553-564 (2018).

Chen, D., Clark, AT, "Mitochondrial DNA Selection in Human Germ", Nature Cell Biology 20: 118-120 (2018).

Gupta, S., Sivalingam, D., Hain, S., Makkar, C., Sosa, E., Clark, AT., Butler, SJ, "Deriving Dorsal Spinal Sentory Interneurons from Human Pluripotent Stem Cells", Stem Cell Reports 10: 390-405 (2018).

Gell, JJ., Zhao, J., Chen, D., Hunt, TJ., Clark, AT, "PRDM14 is expressed in germ cell tumors with overepression altering human germline differentiation and proliferation", Stem Cell Research 27: 46-56 (2018).

Tao, Y., Yen, M-R., Chitiashvili, T., Nakano, H., Kim, R., Hosohama, L., Tan, YC, Nakano, A., Chen, P-Y., Clark, AT, "Trim28-regulated transposon repression is required for human germline competency and not primed or naive pluripotency", Stem Cell Reports 10: 243-256 (2018).

Chen, D., Liu, W., Lukianchikov, A., Hancock, G., Zimmerman, J., Lowe, M., Kim, R., Galic, Z., Irie, N., Surani, MA., Jacobsen, SE., Clark, AT, "Germline competency of human embryonic stem cells depends on eomesodermin", Biology of Reproduction 97: 850-861 (2018).

Chen, D., Gell, JJ., Tao, Y., Sosa, E Clark, AT, "Modeling Infertility with pluripotent stem cells Stem Cell Research", Stem Cell Research 21: 187-192 (2017).

Clark, AT*., Gkountela, G., Chen, D., Liu, W., Sosa, E., Sukhwani, M., Hennebold, JD, Oriwg, KE, "Primordial germ cells acquire transplantation potential by Carnegie Stage 23", Stem Cell Reports 1-13 (2017).

Sosa E, Kim R, Rojas EJ, Hosohama L, Hennebold JD, Orwig KE, Clark AT, "An integration-free visus-free rhesus macaque induced pluripotent stem cell line (iPSC90) from embryonic fibroblasts", Stem Cell Research 21: 5-8 (2017).

Nagaraj R, Sharpley MS, Chi F, Braas D, Zhou Y, Kim R, Clark AT, Banerjee U., "Nuclear localization of mitochondrial TCA cycle enzymes as a critical step in mammalian zygotic genome activation", Cell 168: 210-223 (2017).

O'Brien CM, Chy HS, Zhou Q, Blumenfeld S, Lambshead JW, Liu X, Kie J, Capaldo BD, Chung TL, Adams TE, Phan T, Bentley JD, Mckinstry WJ, Oliva K, Mcmurrick PJ, Wang YC, Rossello FJ, Lindeman GJ, Chen D, Jarde T, Clark AT, Abud HE, Visvader JE, Nefzger CM, Polo JM, Loring JF, Laslett AL., "New monoclonal antibodies to defined cell surface proteins on human pluripotent stem cells", Stem Cells 35: 626-640 (2017).

Patel S, Bonora G, Sahakyan A, Kim R, Chronis C, Langerman J, Fitz-Gibbon S, Rubbi L Skelton RJP, Ardehali R, Pellegrini M, Lowry WE, Clark AT, Plath K, "Human embryonic stem cells do not change their X inactivation status during differentiation", Cell Reports 18: 54-67 (2017).

Sahakyan A, Kim R, Chronis C, Sabri S, Bonora G, Theunissen TW, Kuoy E, Langerman J, Clark AT, Jaenisch R, Plath K, "Naive Human pluripotent stem cells model X chromosome Dampening and X inactivation", Cell Stem Cell 20: 87-101 (2017).

Masaeli M, Gupta D, O'Byrne S, Tse HT, Gossett DR, Tseng P, Utada AS, Jung HJ, Young S, Clark AT, Di Carlo D., "Multiparameter mechanical and morphometric screening of cells", Scientific Reports 6: (2016).

Hargan-Calvopina J, Taylor S, Cook H, Hu Z, Lee SA, Yen M-R, Chiang, Y-S, Chen P-Y and Clark AT, "Stage-specific demethylation in primordial germ cells safeguards against precocious differentiation", Developmental Cell 39: 75-86 (2016).