Mon, 04/25/2016 - 12:00pm
Hershey Hall, Room 158
This seminar is sponsored by: 
UCLA Department of Molecular, Cell, and Developmental Biology and the Institute for Quantitative and Computational Biosciences
Genomic Analysis Laboratory & Plant Biology Laboratory The Salk Institute for Biological Studies

transcription factors (TFs) to capture native genomic DNA. We applied DAPseq to 1,812 Arabidopsis thaliana TFs to resolve motifs for 529 factors and genome-wide enrichment maps for 349 factors. Cumulatively, the ~2.7 million experimentally determined TFBSs captured the Arabidopsis cistrome and predicted thousands of TF target genes enriched for known and novel functions. Base-resolution epicistrome maps were established by comparison of TF-binding to genomic DNA with native cytosinemethylation patterns and genomic DNA that had been synthetically demethylated. This revealed methylcytosine inhibited binding of ~72% of factors and promoted binding of 4.3% of factors. Lastly, we showed DAP-seq binding sites provided a way to annotate genomic and epigenomic variations in natural populations and interpret results from genome-wide association studies. Overall, DAP-seq enables rapid development of base-resolution cistrome and epicistrome atlases for a wide-array of applications for eukaryotic genomes.