Luisa Iruela-Arispe

phone: (310) 794-5763
office: 445 OHRC
lab website

Research Interests

Endothelial cells are unique in their ability to switch from a "differentiated" (non-dividing, non-invasive) to a relatively "undifferentiated" (invasive and highly mitotic) phenotype during the process of blood vessel formation or angiogenesis. We are intrigued by this plasticity and our research efforts are guided towards understanding the molecular mechanisms involved in the regulation of endothelial gene expression. It is hoped that understanding the regulation of this switch will teach us lessons about differentiation pathways in general. Under this umbrella, the major focus of the laboratory has been the dissection of genetic programs involved in the formation and involution of blood vessels during development and in pathological conditions.

In the projects related to developmental angiogenesis, our models include the murine mammary gland and the chicken embryo. The mammary gland provides us with an excellent platform in which to study the expansion and regression of the vasculature associated with the physiological changes of this organ. The chicken embryo enables us to study the formation and remodeling of the vasculature during normal development. Using these two models we search for genes involved in vascular morphogenesis and ask funtional/mechanistic questions using genetics (transgenics and knock-outs in the case of the mouse) and molecular biology (transfection or chicken embryos using electroporation). By perturbing gene expression we hoped to further dissect the molecular pathways that guide vascular formation and remodeling.

A second focus of the lab has been to identify novel angiogenesis inhibitors. Evolution of several pathologies, including the growth of tumors, requires formation of new blood vessels. Blockade of these new blood vessels had been extremely effective in the restriction of tumor growth and suppression of metastasis. Therefore, the identification of novel, tumor-vascular specific, and non-toxic inhibitors has become a major focus of several academic and biotechnology groups. Using the anti-angiogenic region of thrombospondin-1 we have cloned and characterized two novel inhibitors METH-1 (also known as ADAMTS-1) and METH-2 (also known as ADAMTS-8). METH-1 and 2 are able to effectively suppress proliferation of new capillaries and in xenograph assays inhibit the growth of tumors. Both molecules are secreted matrix metalloproteases with disintegrin and thrombospondin motifs. We have invested a great effort in the full characterization and in understanding the mechanisms of action of these molecules. Projects include structure/function analysis of each of the domains: generation of transgenics and knock-ins, identification of substrates / natural inhibitors, and understanding regulation of gene expression.

The effect of steroid hormones, in particular progesterone, on vascular networks in vivo is another focus of the lab. We have found that progesterone inhibits endothelial cell proliferation and have remarkable effects in vascular permeability. Determination of the signaling pathways that result in these events and their biological relevance are major research efforts on this project.

Selected Publications

Keklikoglou, I., CIanciaruso, C., Guc, E., Squadrito, M.L., Spring, L.M., Tazzyman, S., Lambien, L., Poissonnier, A., Ferraro, G.B., Baer, C., Cassara, A., Guichard, A., Iruela-Arispe, M.L., Lewis, C.E., Coussens, L.M., Bardia, A., Jain, R.K., Pollard, J.W., De Palma, M., "Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models", Nature Cell Biol (2018).

Iruela-Arispe, M.L., "Pumping blood with self-reliance and cooperation", Journal of Experimental Medicine 215 (10): 2480-2482 (2018).

Iruela-Arispe, M.L., "A dual origin for blood vessels", Nature 562 (7726): 195-197 (2018).

McDonald, A.I., Shirali, A.S., Arag?n, R., Ma, F., Hernandez, G., Vaughn, D.A., Mack, J.J., Lim, T.Y., Sunshine, H., Zhao, P., Kalinichenko, V., Hai, T., Pelegrini, M., Ardehali, R., and Iruela-Arispe, M.L., "Endothelial Regeneration of Large Vessels is a Biphasic Process Driven by Local Cells with Distinct Proliferative Capacities", Cell Stem Cell 23 (2): 210-225 (2018).

Archer, B., Ueberrueck, T., Mack, J., Yousef, K., Jarenwattananon, N.N., Rall, D., Wypysek, D., Wiese, M., Bluemich, B., Wessling, M., Iruela-Arispe, L., and Bouchard, L., "Non-Invasive Quantification of Cell Density in 3D Gels by MRI", IEEE Trans Biomed Eng 1-10 (2018). [link]

Jelinek, D., Flores, A., Uebelhoer, M., Pasque, V., Plath, K., Iruela-Arispe, M.L., Christofk, H.R., Lowry, W.E., and Coller, H.A., "Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue", J Vis Exp (2018). [link]

Chattopadhyay, A., Yang, X., Mukherjee, P., Sulaiman, D., Fogelman, H.R., Grijalva, V., Dubinett, S., Wasler, T.C., Paul, M.K., Salehi-Rad, R., Mack, J.J., Iruela-Arispe, M.L., Navab, M., Fogelman, A.M., and Reddy, S.T., "Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung Cancer", 8 (1): 9032-9043 (2018).

Hilfenhaus, G., Nguyen, D., Freshman, J., Prajapati, D., Ma, F., Song, D., Ziyad, S., Cuadrado, M., Pellegrini, M., Bustelo, X.R., and Iruela-Arispe, M.L., "VAV3-induced Cytoskeletal Dynamics Contribute to Heterotypic Properties of Endothelial Barriers", J Cell Biol 217 (8): 2813-2830 (2018).

Nowak-Sliwinska, P., Alitalo, K., Allen, A., Anisimov, A., Aplin, A.C., Auerbach, R., Augustin, A.G., Bates, D.O., van Beijnum, J.T., Bender, R.H.,F., Bergers,G., Bikfalvi, A., Bischoff, J., Böck B.C., Brooks, P.C., Bussolino, F., Cakir, B., Carmeliet, P., Castranova, D., Cimpean, A.M., Cleaver, O., Coukos, G., Davis, G.E., De Palma, M., Dimberg, A., Dings, R.P.M., Djonov, V., Dudley, A.C., Dufton, N.P., Fendt, S.M., Ferrara, N., Fruttiger, M., Fukumura, D., Ghesquière, B., Gong, Y., Griffin, R.J., Harris, A.L., Hughes, C.C.W., Hultgren, N.W., Iruela-Arispe, M.L., Irving, M., Jain, R.K., Kalluri, R., Kalucka, J., Kerbel, R.S., Kitajewski, J., Klaassen, I., Kleinmann, H.K., Koolwijk, P., Kuczynski, E., Kwak, B.R., K. Marien, J. M. Melero-Martin, L. L. Munn, R. F. Nicosia, A. Noel, J. Nurro, A.-K. Olsson, T. V. Petrova, Pietras, K., Pili, R., Pollard, J.W., Post, M.J., Quax, P.H.A., Rabinovich, G.A., Raica, M., Randi, A.M., Ribatti, D., Ruegg, C., Schlingemann, R.O., Schulte-Merker, S., Smith, L.E.H., Song, J.W., Stacker, S.A., Stalin, J., Stratman, A.N., Van de Velde, M., van Hinsbergh, V.W.M., Vermeulen, P.B., Waltenberger, J., Weinstein, B.M., Xin, H., Yetkin-Arik, B., Yla-Herttuala, S., Yoder, M.C., and Griffioen, A.W., "Consensus guidelines for the use and interpretation of angiogenesis assays", Angiogenesis 21 (3): 425-532 (2018).

Mack, J.J., and Iruela-Arispe, M.L., "NOTCH regulation of the endothelial cell phenotype", Current Opinion in Hematology 25 (3): 212-218 (2018).

Freitas, V.M., Hilfenhaus, G., and Iruela-Arispe, M.L., "Metastasis of Circulating Tumor Cells: Speed Matters", Developmental Cell 45 (1): 3-5 (2018).

Shirali, A.S., Romay, M.C., McDonald, A.I., Su, T., Steel, M.E., and Iruela-Arispe, M.L., "A multi-step transcriptional cascade underlies vascular regeneration in vivo", Scientific Reports 8 (1): 1-17 (2018).

Li, L., Zeng, Q., Bhutkar, A., Galvan, J., Karamitopoulou, E., Necsulea, A., Noordermeer, D., Piersigilli, A., Peng, M., Perren, A., Ziobec, I., Robinson, H., Iruela-Arispe, M.L., and Hanahan, D., "GKAP acts as a genetic modulator of NMDAR signaling to govern invasive tumor growth", Cancer Cell 33 (4): 736-751 (2018). [link]

Sereti, K.I., Nguyen, N.B., Kamran, P., Zhao, P., Ranjbarvaziri, S., Park, S., Sabri, S., Engel, J.L., Sung, K., Kulkarni, R.P., Ding, Y., Hsiai, T.K., Plath, K., Ernst, J., Sahoo, D., Mikkola, H.K.A., Iruela-Arispe, M.L., and Ardehali, R., "Analysis of cardiomyocyte clonal expansion during mouse heart development and injury", Nature Communications 9 (1): 1-13 (2018).

Ziyad, S., Riordan, J.D., Cavanaugh, A.C., Su, T., Hernandez, G.E., Hilfenhaus, G., Morselli, M., Huynh, K., Wang, K., Chen, J.N., Dupuy, A.J., and Iruela-Arispe, M.L., "A forward genetic screen targeting the endothelium reveals a regulatory role for the lipid kinase Pi4Ka in myelo- and erythropoiesis", Cell Reports 22 (5): 1211-1224 (2018).